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hiscript iii reverse transcriptase kit  (Vazyme Biotech Co)


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    Structured Review

    Vazyme Biotech Co hiscript iii reverse transcriptase kit
    Hiscript Iii Reverse Transcriptase Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 96/100, based on 719 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hiscript+iii+reverse+transcriptase+kit/Hiscript+III+Reverse+Transcriptase/pm41879300-227-20-25
    Average 96 stars, based on 719 article reviews
    hiscript iii reverse transcriptase kit - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    RNA Extraction:

    Article Title: A novel and evolutionarily distinct flavoprotein monooxygenase drives skatole degradation in Rhodococcus .
    Article Snippet: .. Total RNA was extracted from bacterial cultures using a Vazyme RNA extraction kit and reverse-transcri bed into cDNA using the Hiscript III Reverse Transcriptase kit (Vazyme, China). qPCR reactions were performed using ChamQ Universal SYBR qPCR Master Mix (Vazyme, China) on a real-time PCR system (LightCycler 480II, Roche, Switzerland). ..

    Article Title: Plasmodium yoelii infection induces lung injury by modulating type 2 conventional dendritic cells autophagy via the STAT3-IRF4 signaling.
    Article Snippet: .. RNA was extracted with a SteadyPure Universal RNA Extraction kit II (Accurate Biotechnology, China), and cDNA was synthesized with a HiScript III reverse transcriptase kit (Vazyme, China). .. Real-time PCR amplification was carried out in the presence of 1 μL of cDNA template, 5 μL of SYBR master mixture (Vazyme, China), and target gene-specific primers (Table S1) in a CFX Connect Real-time PCR Detection System (BioRad, CA).

    Article Title: A novel and evolutionarily distinct flavoprotein monooxygenase drives skatole degradation in Rhodococcus
    Article Snippet: .. Total RNA was extracted from bacterial cultures using a Vazyme RNA extraction kit and reverse-transcribed into cDNA using the Hiscript III Reverse Transcriptase kit (Vazyme, China). qPCR reactions were performed using ChamQ Universal SYBR qPCR Master Mix (Vazyme, China) on a real-time PCR system (LightCycler 480II, Roche, Switzerland). ..

    Reverse Transcription:

    Article Title: A novel and evolutionarily distinct flavoprotein monooxygenase drives skatole degradation in Rhodococcus .
    Article Snippet: .. Total RNA was extracted from bacterial cultures using a Vazyme RNA extraction kit and reverse-transcri bed into cDNA using the Hiscript III Reverse Transcriptase kit (Vazyme, China). qPCR reactions were performed using ChamQ Universal SYBR qPCR Master Mix (Vazyme, China) on a real-time PCR system (LightCycler 480II, Roche, Switzerland). ..

    Article Title: BCAA catabolism mediates POU2AF1 propionylation to enhance T-ALL development.
    Article Snippet: Total RNA from T-ALL cells was extracted with the FastPure® Cell/Tissue Total RNA Isolation Kit V2 (Vazyme, #RC112). .. The obtained RNA was treated with gDNA Wiper and reverse transcribed into cDNA using the HiScript III Reverse Transcriptase kit (Vazyme, #R323). .. Quantitative real-time PCR (RT-qPCR) was carried out with ChamQ SYBR qPCR Master Mix (Vazyme, #Q341-02), and fluorescence signals were measured on a Bio-Rad CFX96 Touch Real-Time PCR Detection System.

    Article Title: Glutathione peroxidase mimic-engineered α-lactalbumin self-assembling coatings tailor immunomodulatory vascular stents for suppressing restenosis
    Article Snippet: .. Total RNA was extracted using TRIzolTM reagent (TSP401, Tsingke Biotechnology, China), and first-strand cDNA was synthesized using the HiScript III Reverse Transcriptase Kit (Vazyme, China) according to the manufacturer's instructions. .. Quantitative PCR was performed in triplicate using the QuantStudioTM 3 Real-Time PCR System (Thermo Fisher Scientific, USA).

    Article Title: BCAA catabolism mediates POU2AF1 propionylation to enhance T-ALL development
    Article Snippet: Total RNA from T-ALL cells was extracted with the FastPure ® Cell/Tissue Total RNA Isolation Kit V2 (Vazyme, #RC112). .. The obtained RNA was treated with gDNA Wiper and reverse transcribed into cDNA using the HiScript III Reverse Transcriptase kit (Vazyme, #R323). .. Quantitative real-time PCR (RT-qPCR) was carried out with ChamQ SYBR qPCR Master Mix (Vazyme, #Q341-02), and fluorescence signals were measured on a Bio-Rad CFX96 Touch Real-Time PCR Detection System.

    Article Title: Plasmodium yoelii infection induces lung injury by modulating type 2 conventional dendritic cells autophagy via the STAT3-IRF4 signaling.
    Article Snippet: .. RNA was extracted with a SteadyPure Universal RNA Extraction kit II (Accurate Biotechnology, China), and cDNA was synthesized with a HiScript III reverse transcriptase kit (Vazyme, China). .. Real-time PCR amplification was carried out in the presence of 1 μL of cDNA template, 5 μL of SYBR master mixture (Vazyme, China), and target gene-specific primers (Table S1) in a CFX Connect Real-time PCR Detection System (BioRad, CA).

    Article Title: Construction and validation of a novel prognostic signature that correlates with immune infiltration based on glycolysis related lncRNAs in osteosarcoma.
    Article Snippet: Total RNA was extracted using Trizol reagent (Vazyme, Nanjing, China) following the manufacturer’s protocols. .. Then, RNA samples were reverse transcribed by Hiscript III Reverse Transcriptase kit (Vazyme, Nanjing, China) and the corresponding RNA expression was evaluated by qRT-PCR with ChamQTM Universal SYBR qPCR Master Mix kit (Vazyme, Nanjing, China). ..

    Article Title: A novel and evolutionarily distinct flavoprotein monooxygenase drives skatole degradation in Rhodococcus
    Article Snippet: .. Total RNA was extracted from bacterial cultures using a Vazyme RNA extraction kit and reverse-transcribed into cDNA using the Hiscript III Reverse Transcriptase kit (Vazyme, China). qPCR reactions were performed using ChamQ Universal SYBR qPCR Master Mix (Vazyme, China) on a real-time PCR system (LightCycler 480II, Roche, Switzerland). ..

    Article Title: Enalaprilat reverses neutrophil polarization imbalance via targeting taurine-STING axis for treatment of diabetic wounds
    Article Snippet: FerroOrange assay kit , Dojindo , Cat#F374. .. Hiscript III reverse transcriptase kit , Vazyme , Cat#R302-01. .. Matrigel matrix , Corning , Cat#356234.

    Real-time Polymerase Chain Reaction:

    Article Title: A novel and evolutionarily distinct flavoprotein monooxygenase drives skatole degradation in Rhodococcus .
    Article Snippet: .. Total RNA was extracted from bacterial cultures using a Vazyme RNA extraction kit and reverse-transcri bed into cDNA using the Hiscript III Reverse Transcriptase kit (Vazyme, China). qPCR reactions were performed using ChamQ Universal SYBR qPCR Master Mix (Vazyme, China) on a real-time PCR system (LightCycler 480II, Roche, Switzerland). ..

    Article Title: Construction and validation of a novel prognostic signature that correlates with immune infiltration based on glycolysis related lncRNAs in osteosarcoma.
    Article Snippet: Total RNA was extracted using Trizol reagent (Vazyme, Nanjing, China) following the manufacturer’s protocols. .. Then, RNA samples were reverse transcribed by Hiscript III Reverse Transcriptase kit (Vazyme, Nanjing, China) and the corresponding RNA expression was evaluated by qRT-PCR with ChamQTM Universal SYBR qPCR Master Mix kit (Vazyme, Nanjing, China). ..

    Article Title: A novel and evolutionarily distinct flavoprotein monooxygenase drives skatole degradation in Rhodococcus
    Article Snippet: .. Total RNA was extracted from bacterial cultures using a Vazyme RNA extraction kit and reverse-transcribed into cDNA using the Hiscript III Reverse Transcriptase kit (Vazyme, China). qPCR reactions were performed using ChamQ Universal SYBR qPCR Master Mix (Vazyme, China) on a real-time PCR system (LightCycler 480II, Roche, Switzerland). ..

    Synthesized:

    Article Title: Glutathione peroxidase mimic-engineered α-lactalbumin self-assembling coatings tailor immunomodulatory vascular stents for suppressing restenosis
    Article Snippet: .. Total RNA was extracted using TRIzolTM reagent (TSP401, Tsingke Biotechnology, China), and first-strand cDNA was synthesized using the HiScript III Reverse Transcriptase Kit (Vazyme, China) according to the manufacturer's instructions. .. Quantitative PCR was performed in triplicate using the QuantStudioTM 3 Real-Time PCR System (Thermo Fisher Scientific, USA).

    Article Title: Plasmodium yoelii infection induces lung injury by modulating type 2 conventional dendritic cells autophagy via the STAT3-IRF4 signaling.
    Article Snippet: .. RNA was extracted with a SteadyPure Universal RNA Extraction kit II (Accurate Biotechnology, China), and cDNA was synthesized with a HiScript III reverse transcriptase kit (Vazyme, China). .. Real-time PCR amplification was carried out in the presence of 1 μL of cDNA template, 5 μL of SYBR master mixture (Vazyme, China), and target gene-specific primers (Table S1) in a CFX Connect Real-time PCR Detection System (BioRad, CA).

    RNA Expression:

    Article Title: Construction and validation of a novel prognostic signature that correlates with immune infiltration based on glycolysis related lncRNAs in osteosarcoma.
    Article Snippet: Total RNA was extracted using Trizol reagent (Vazyme, Nanjing, China) following the manufacturer’s protocols. .. Then, RNA samples were reverse transcribed by Hiscript III Reverse Transcriptase kit (Vazyme, Nanjing, China) and the corresponding RNA expression was evaluated by qRT-PCR with ChamQTM Universal SYBR qPCR Master Mix kit (Vazyme, Nanjing, China). ..

    Quantitative RT-PCR:

    Article Title: Construction and validation of a novel prognostic signature that correlates with immune infiltration based on glycolysis related lncRNAs in osteosarcoma.
    Article Snippet: Total RNA was extracted using Trizol reagent (Vazyme, Nanjing, China) following the manufacturer’s protocols. .. Then, RNA samples were reverse transcribed by Hiscript III Reverse Transcriptase kit (Vazyme, Nanjing, China) and the corresponding RNA expression was evaluated by qRT-PCR with ChamQTM Universal SYBR qPCR Master Mix kit (Vazyme, Nanjing, China). ..



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